Composite
Part:BBa_K4129133:Design
Designed by: Magnus Haahr Group: iGEM22_DTU-Denmark (2022-10-10)
The fungal synthetic expression system with FunsTF70 and 6xLexO-Pmin
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4186
Illegal XbaI site found at 6099
Illegal PstI site found at 141 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 911
Illegal NheI site found at 2986
Illegal PstI site found at 141 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 145
Illegal BglII site found at 3173
Illegal BamHI site found at 2920
Illegal BamHI site found at 5380
Illegal XhoI site found at 701
Illegal XhoI site found at 1083
Illegal XhoI site found at 4050 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4186
Illegal XbaI site found at 6099
Illegal PstI site found at 141 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4186
Illegal XbaI site found at 6099
Illegal PstI site found at 141
Illegal NgoMIV site found at 3078
Illegal NgoMIV site found at 6016
Illegal AgeI site found at 1290
Illegal AgeI site found at 1438 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1117
Illegal BsaI.rc site found at 5730
Illegal SapI site found at 323
Design Notes
The assembly was standardised to assemble the cassettes in bulk.
Source
The individual parts were either salvaged from in-house plasmids or de novo synthesised: